When
Abstract: Lysosomes and mitochondria are membrane-bound organelles in the cell that are responsible for trash-disposal and ATP production, respectively. Lysosomal and mitochondrial dysfunctions have been linked to numerous human diseases, such as neurodegeneration, cancer, and cardiovascular diseases. These organelles are highly dynamic and frequently interact with other cellular components in a transient fashion, which are difficult to capture with traditional immunoprecipitation and organelle isolation methods. We have developed and improved several proximity labeling proteomic approaches to characterize sub-organelle microenvironment and protein networks in live cells. Here, I will describe our recent efforts in developing proximity labeling proteomic methods and understanding neurodegenerative diseases in patient-derived fibroblasts and iPSC-derived neuron platforms. I will also introduce our newly established universal and sample type-specific contaminant libraries that can benefit the broad proteomics community.
Bio-sketch: Dr. Ling Hao is an Assistant Professor in the Chemistry Department at the George Washington University in Washington, D.C. She received her PhD from the University of Wisconsin-Madison followed by a postdoctoral fellowship at the National Institutes of Health (NIH) before joining GW in 2019. She has co-authored over 30 publications with 1900 citations and served as a reviewer for 15 research journals and a guest editor for the Journal of Visualized Experiments. She has received a Ralph E Powe Junior Faculty Enhancement Award from Oak Ridge Associated Universities (ORAU), a 5-year NIH R01 grant, and a GW UFF grant to support her research in developing mass spectrometry-based bioanalytical chemistry methods to study brain diseases. She was also recognized as an Emerging Investigator by the Journal of American Society for Mass Spectrometry in 2021.
LOCATION
Koffler - Room 218
PRESENTER
Dr. Ling Hao Assistant Professor, Department of Chemistry, George Washington University
Hosted byDr. Craig Aspinwall