CBC Colloquium:"Enhanced DNA Bending/Unwinding Fluctuations Promote Damage Recognition by Rad4/XPC DNA-Repair Complex"Thursday, February 21, 2019 - 4:00pm
DNA damage repair starts with the recognition of damaged sites from predominantly normal DNA. In eukaryotes, diverse DNA lesions from environmental sources are recognized by the XPC nucleotide excision repair complex. Studies with Rad4 (yeast XPC ortholog) showed that Rad4 “opens” up damaged DNA sites by inserting a -hairpin into the duplex and flipping out two damage-containing nucleotide pairs. However, characterizing the dynamics of these conformational changes along the lesion recognition trajectory has been challenging.