Research Assistant Professor, Chemistry and Biochemistry - Med
Degrees and Appointments
- Ph.D. (Medicine) 2006, National University of Singapore
The serine/threonine phosphatase Protein Phosphatase 1 (PP1) is involved in many essential biological processes, such as protein translation, cell cycle, blood glucose level and memory formation. The diversity of the functions of PP1 is achieved through its interactions with over 200 regulatory subunits, each one targeting PP1 to a specific substrate, thus enabling it to control different cell signaling pathways. My research for the past 10 years has been focusing on elucidating at the molecular level how PP1 regulators differentially regulate PP1. I am also interested in identifying new PP1 binding motifs and designing small molecule inhibitors that specifically target PP1.
- X-ray crystallography of protein complexes
- Fragment based screening and drug discovery
-Protein expression and purification from bacteria, insect and mammalian cells
Choy MS, Bolik-Coulon N, Archuleta TL, Peti W, Page R (2018). The structure of SDS22 provides insights into the mechanism of heterodimer formation with PP1. Acta Crystallographica F, 74 (Pt 12):817-824. PMCID: PMC6277963
Kumar GS, Choy MS, Koveal DM, Lorinsky MK, Lyons SP, Kettenbach AN, Page R, Peti W (2018). Identification of the substrate recruitment mechanism of the muscle glycogen protein phosphatase 1 holoenzyme. Science Advances, 4:eaau6044. PMCID: PMC6235537
Choy MS, Swingle M, D’Arcy B, Abney K, Rusin SF, Kettenbach AN, Page R, Honkanen RE, Peti W (2017). PP1:tautomycetin complex reveals a path toward the development of PP1-specific inhibitors. Journal of American Chemical Society, 139(49), 17703 – 17706.
Choy MS, Li Y, Machado LESF, Kunze MBA, Connors CR, Wei X, Lindorff-Larsen K, Page R, Peti W (2017). Conformational Rigidity and Protein Dynamics at Distinct Timescales Regulate PTP1B Activity and Allostery. Molecular Cell, 65(4):644 – 658. PMCID: PMC5325675
Krishnan N, Krishnan K, Connors CR, Choy MS, Page R, Peti W, Van Aelst L, Shea SD, Tonks NK. PTP1B inhibition suggests a therapeutic strategy for Rett syndrome. (2015) Journal of Clinical Investigation, 125(8):3163 – 3177. PMCID: PMC4563751
Chen E, Choy MS, Petrényi K, Kónya Z, Erdődi F, Dombrádi V, Peti W, Page R (2016). Molecular Insights into the fungus-specific serine/threonine protein phosphatase Z1 in Candida albicans. mBio, 7(4):e00872-16. PMCID: PMC4999541
Choy MS, Yusoff P, Lee IC, Newton JC, Goh CW, Page R, Shenolikar S, Peti W. (2015) Structural and Functional Analysis of the GADD34:PP1 eIF2α Phosphatase. Cell Reports, 11(12):1885 – 1891. PMCID: PMC4489983
Choy MS, Hieke M, Kumar GS, Lewis GR, Gonzalez-DeWhitt KR, Kessler RP, Stein BJ, Hessenberger M, Nairn AC, Peti W, Page R (2014). Understanding the antagonism of retinoblastoma protein dephosphorylation by PNUTS provides insights into the PP1 regulatory code. Proceedings of the National Academy of Sciences, 111(11):4097 – 4102. PMCID: PMC3964120
Choy MS, Page R, Peti W (2012). Regulation of protein phosphatase 1 by intrinsically disordered proteins. Biochemical Society Transaction. 2012 Oct; 40(5):969-74. PMCID: PMC3502941