"Organic Qualitative Analysis" (Spring Semester) is a laboratory course for Chemistry majors focused on methods for identification of unknown organic compounds, with a major emphasis on NMR. Students are trained on the Varian Gemini-200 NMR instrument (full hands-on operation) and the Bruker AVIII-400 (automation including 2D NMR) and have open-access hands-on use of the instruments all semester for analysis of unknowns by 1H, 13C, DEPT, COSY, HSQC and HMBC. In the second half of the semester, students analyze an unknown steroid using the Bruker AVIII-400 as well as a complete set of 2D NMR data acquired on a 600-MHz instrument. The 2D data is supplied as many pages of printouts of the DQF-COSY, HSQC and HMBC spectra. A series of lectures teaches the interpretation of these 2D spectra and the reasoning used to determine structure (connectivity of atoms) from the 1D and 2D NMR data.
Currently there are three graduate courses which teach theory and practice of Nuclear Magnetic Resonance Spectroscopy: 1) Chemistry 543, "Organic Spectroscopy" (Fall Semester) 2) Chemistry 534A and 534B "Practical NMR Spectroscopy Laboratory" and "Practical NMR Spectroscopy" (Spring Semester, 534A odd-numbered years, 534B even-numbered years) 3) Chemistry 584 "NMR Spectroscopy" (Fall Semester)
1) Chemistry 545 is required for all first year Chemistry graduate students in the Organic Division. There are two portions of the course: Organic Synthesis and Organic Spectroscopy. The laboratory is focused on learning modern techniques for organic synthesis, using NMR as the primary tool for analysis of mixtures and synthetic products. One 50-minute lecture per week is devoted to the spectroscopy portion; most of these are on 1D and 2D NMR data interpretation. All students will be trained on the Bruker AVIII-400, DRX-500 and DRX-600 NMR instruments.
2a) Chemistry 534A is a laboratory course in modern NMR techniques, focusing on the commonly used experiments for organic structure elucidation in the solution state: 1H, 13C, DEPT, 1D NOE, 1D TOCSY and 2D COSY, HSQC, HMBC, NOESY, ROESY and TOCSY. Theory is presented at a level sufficient to understand how the experiments work and how to optimize the parameters for best results. Interpretation of spectra is presented in great detail, with many examples and problem sets. Finally, NMR hardware is discussed in general with block diagrams to understand how the modern NMR instrument works. Each student has 2 hours per week on an NMR spectrometer (Varian Unity-300, Bruker DRX-500, and Varian Inova-600) and determines the structure of a complex organic compound. Textbook: NMR Spectroscopy Explained, Neil E. Jacobsen, Wiley, 2007.
2b) Chemistry 534B is a lecture course in modern NMR techniques. Because there is no laboratory, more time is spent on NMR theory and we go into more detail in providing a description of each experiment using the vector model, the product operator formalism, and touching briefly on the matrix representation. Theory is limited to spin-1/2 nuclei (especially 1H and 13C) in the solution state. The material covered is similar to that of Chemistry 534A, but both courses may be taken in any sequence for a total of 6 credits. Textbook: NMR Spectroscopy Explained, Neil E. Jacobsen, Wiley, 2007.
3) Chemistry 584 is a lecture course focusing on NMR in chemistry, biology and physics research. It is part of the graduate program for the Physical Chemistry Division of the Chemistry Department, with cross-listings in the Biochemistry and Physics Departments. The course has a broader focus than Chemistry 534, including solid-state NMR and NMR imaging as well as liquid-state NMR, and the theory is presented with a more complete mathematical formalism. Textbook: Spin Dynamics, Basics of Nuclear Magnetic Resonance, Malcolm H. Levitt, Wiley, 2001.